Isolation And Characteristics Of Lactic Acid Bacteria In Feces Of Jember Local Mongoose

Authors

  • Kukuh Munandar Universitas Muhammadiyah Jember
  • Dina Afriayanti Universitas Muhammadiyah Jember
  • Inayatul Karimah Universitas Muhammadiyah Jember

DOI:

https://doi.org/10.32528/ias.v1i1.46

Keywords:

bacteria, lactic acid bacteria, mongoose (Paradoxurus hermaphroditus)

Abstract

Drinking a cup of coffee is not only a matter of taste but also a part of Indonesian’s daily life. Coffee shops which have proliferated so far proves this lifestyle. One of the best selling coffee bean products is mongoose coffee. It is a product of coffee beans which have been swallowed by mongoose and through its digestive tract. In the digestive tract, beans are being fermented by microbes (lactic acid bacteria or LAB). This fermentation process contributes to the unique taste and aroma of the coffee. The taste and aroma are very appealing to coffee lovers, resulting in the rise of demands in both local and international markets in every year. However, this leads to the escalation of mongoose hunting, threatening the population of mongoose (Paradoxurus hermaphroditus). Moreover, its limited digesting capacity also hinders the production of mongoose coffee. The present study offers an alternative to produce mongoose coffee by optimizing in-vitro fermentation which simulates the natural process of fermentation inside mongoose’s digestive organs. This in-vitro fermentation uses bacteria isolate (LAB) from mongoose’s feces collected from the local society of Jember. The study, therefore, aimed to: 1) isolate LAB from mongoose’s feces, and 2) identify the characteristics of collected LAB. Based on the result of data analysis, it was concluded that 5 LAB isolates from local mongoose’s feces were identified, namely: LAB-1 is Lactobacillus plantarum, LAB-2 is Lactobacillus brevis, LAB-3 is Leuconostoc paramesenteroides, LAB-4 is Leuconostoc mesenteroides, and LAB-5 is Streptococcus faecium

Downloads

Download data is not yet available.

References

Cheyne, S. M. et al. (2010). Diversity and activity of small carnivores of the Sabangau Peat-swamp Forest, Indonesian Borneo. Small Carnivore Conservation, 43(December 2010): 1–7.

Rahayu, S., Rahmawati, dan R. Kurniatuhadi (2018). Deteksi Bakteri Selulolitik pada Kotoran Luwak (Paradoxurus hermaphroditus) dari Kebun Binatang Bandung. Protobiont, 7(2): 19 – 28

Chan, S., and E. Garcia (2011).Comparative Physicochemical Analyses of Regular and Civet Coffee. The Manila Journal of Science, 7(1): 19-23.

Suhandy, D. and M. Yulia (2017).The Use of Partial Least Square Regression and Spectral Data in UV-Visible Region for Quantification of Adulteration in Indonesian Palm Civet Coffee. International Journal of Food Science, Volume 2017, Article ID 6274178, 7 pages, https://doi.org/10.1155/2017/6274178

Kompas.com (2009). Permintaan Tinggi, Ekspor Kopi Luwak Dibatasi. (daring) https://tekno.kompas.com/read/2009/10/29/18532371/permintaan.tinggi.ekspor.kopi.luwak.dibatasi.

Marcone, MF. (2004) Composition and Properties of Indonesian Palm Civet Coffee (Kopi Luwak) and Ethiopian Civet Coffee. Food Research International, 37 (2004): 901–912 doi:10.1016/j.foodres.2004.05.008

Muzaifa, M., A. Patria, A. Abubakar, F. Rahmi, D. Hasni, I. Sulaiman, dan Febriani (2006). Kopi Luwak: Produksi, Mutu Dan Permasalahannya. Aceh: Syiah Kuala University Press.

Carder, G., H Proctor, J Schmidt-Burbach, and N D’Cruze (2016). The animal welfare implications of civet coffee tourism in Bali. Animal Welfare, 25: 199-205 (doi: 10.7120/09627286.25.2.199).

Shepherd, C.R. (2012).Observations of small carnivores in Jakarta wildlife markets, Indonesia, with notes on trade in Javan Ferret Badger Melogale orientalis and on the increasing demand for Common Palm Civet Paradoxurus hermaphroditus for civet coffee production. Small Carnivore Conservation, Vol. 47: 38–41, December 2012.

Leroy, F. and L. De Vuyst (2004). Lactic acid bacteria as functional starter cultures for the food fermentation industry. Trends in Food Science & Technology, 15 (2004): 67–78.

Ogunyemi, A.., B.C. Ogunyemi, A.L. Ategun, B.A. Iwalokun, J.I. Ike, M.O. Ilori, and O.O. Amund. (2020). Isolation and characterisation of nitrilase producing Aspergillus species recovered from solid waste leachates at two dump sites in Lagos, Nigeria. Malaysian Journal of Microbiology, Vol. 16(3): 2020, pp. 153-158, DOI: http://dx.doi.org/10.21161/mjm.180234

Hassan P., and Peh Kok (2019). Confirmation of the Identity of Lactobacillus Species using Carbohydrate Fermentation Test (API 50 CHL) Identification System. Journal of Applied Sciences, 19(8): 797-802, DOI: 10.3923/jas.2019.797.802

Rahmawati, NHF. (2017). Isolasi dan Karakterisasi Bakteri Proteolitik dari Feses Hewan Luwak (Paradoxurus hermaphroditus). Jurnal Prodi Biologi, 6(1): 1-8

Hadipernata, M. dan S. Nugraha (2012). Identifikasi Fisik, Kimia Dan Mikrobiologi Biji Kopi Luwak Sebagai Dasar Acuan Teknologi Proses Kopi Luwak Artificial. Prosiding InSINas 2012: PG-117-121

Monique Zagorec, Jamila Anba-Mondoloni, Anne-Marie Crutz-Le Coq, and Marie-Christine Champomier-Verg`es (2008). Genetics of Lactic Acid Bacteria. In Toldr´, F. (Ed.) (2008). Meat Biotechnology. (2008). New York: Springer Science+Business Media, LLC.

Valerio, F., M. Favilla, P. De Bellis, A. Sisto, S. de Candia, and P. Lavermicocca (2009). Antifungal activity of strains of lactic acid bacteria isolated from asemolina ecosystem against Penicillium roqueforti, Aspergillus niger and Endomyces fibuliger contaminating bakery products. Systematic and Applied Microbiology, 32 (2009): 438–448

Chakraborty, A. and J. Bhowal (2015). Isolation, Identification and Analysis of Probiotic Properties of Lactobacillus Spp. from Selected Regional Dairy Product. Int.J.Curr.Microbiol.App.Sci, 4(6): 621-628

Pessoa, MG., Vespermann, KAC., Paulino, BN., Barcelos, MCS., Pastore, GM., and Molina, G. (2019). Newly Isolated Microorganisms with Potential Application in Biotechnology. Biotechnology Advances. 37(2): 319-339. DOI: 10.1016/j.biotechadv.2019.01.007

Downloads

Published

2022-01-30

How to Cite

Munandar, K., Afriayanti, D., & Karimah, I. (2022). Isolation And Characteristics Of Lactic Acid Bacteria In Feces Of Jember Local Mongoose. International Applied Science, 1(1), 43–47. https://doi.org/10.32528/ias.v1i1.46